Mouse Complement Factor H Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4999

R&D Systems, part of Bio-Techne
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AF4999
AF4999-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Complement Factor H Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor H
Ser875-Val1252
Accession # NP_034018

Specificity

Detects mouse Complement Factor H in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Complement Factor H is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Complement Factor H Antibody

Detection of Mouse Complement Factor H antibody by Western Blot.

Detection of Mouse Complement Factor H by Western Blot.

Western blot shows lysates of Hepa 1-6 mouse hepatoma cell line, mouse liver tissue, and mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Complement Factor H antibody by Simple WesternTM.

Detection of Mouse Complement Factor H by Simple WesternTM.

Simple Western lane view shows mouse plasma, loaded at 0.2 mg/mL. A specific band was detected for Complement Factor H at approximately 166 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4999) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Complement Factor H Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Mouse plasma

Western Blot

1 µg/mL
Sample: Hepa 1‑6 mouse hepatoma cell line, Mouse liver tissue, and Mouse plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H

Factor H is a 155 kDa glycoprotein that provides critical negative regulation of the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration (1). Factor H is composed of 20 SCR (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functioning as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). This recombinant protein corresponds to SCR15-20 which encompass the primary binding sites for heparin and C3b as well as for the peptide hormone adrenomedullin (4, 9-11). Within SCR15-20, mouse Factor H shares 60% and 80% amino acid sequence identity with human and rat Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement mediated lysis (13, 14). A variety of pathogenic microbes also express Factor H binding molecules that interfere with immune clearance of the infection (15).

References

  1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14.
  2. Kristensen, T. and B.F. Tack (1986) Proc. Natl. Acad. Sci. 83:3963.
  3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. 87:3982.
  4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173.
  5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342.
  6. Hellwage, J. et al. (2002) J. Immunol. 169:6935.
  7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057.
  8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. 93:10996.
  9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
  10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
  11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
  12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
  13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
  14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
  15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.

Alternate Names

ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS

Entrez Gene IDs

3075 (Human); 12628 (Mouse); 155012 (Rat)

Gene Symbol

CFH

UniProt

NP_034018

Additional Complement Factor H Products

Product Documents for Mouse Complement Factor H Antibody

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Certificate of Analysis

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Product Specific Notices for Mouse Complement Factor H Antibody

For research use only

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