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Mouse Contactin-3 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6644N

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 524518

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin‑3
Glu20-Thr1003
Accession # Q07409

Specificity

Detects mouse Contactin‑3 in direct ELISAS. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Contactin-3 and no cross‑reactivity with recombinant mouse (rm) Contactin-1, -4, -6, rhContactin-2 or -5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Contactin-3 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Contactin-3

Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons, and olfactory granule cells. It likely mediates cell-cell interaction and may promote neurite outgrowth. The mouse Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region, and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa 32-593), followed by four Fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, mouse Contactin-3 shares 92% and 96% aa identity with human and rat Contactin-3, respectively.

Alternate Names

BIG-1, CNTN3, Contactin3, PANG, PCS

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Gene Symbol

CNTN3

UniProt

Additional Contactin-3 Products

Product Documents for Mouse Contactin-3 Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Contactin-3 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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