Mouse CTHRC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5960
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CTHRC1
Ser33-Lys245
Accession # Q3UAP6
Ser33-Lys245
Accession # Q3UAP6
Specificity
Detects mouse CTHRC1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CTHRC1 Antibody
Detection of Mouse CTHRC1 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse CTHRC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5960) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTHRC1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
CTHRC1 in Mouse Embryo.
CTHRC1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse CTHRC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5960) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse CTHRC1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)
Western Blot
1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CTHRC1
Long Name
Collagen Triple Helix Repeat Containing 1
Alternate Names
NMTC1
Gene Symbol
CTHRC1
UniProt
Additional CTHRC1 Products
Product Documents for Mouse CTHRC1 Antibody
Product Specific Notices for Mouse CTHRC1 Antibody
For research use only
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