Mouse CX3CL1/Fractalkine APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB571A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 126315
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine
Specificity
Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1 (full length), and 50% cross-reactivity with recombinant rat CX3CL1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CX3CL1/Fractalkine APC-conjugated Antibody
Detection of CX3CL1/Fractalkine in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # FAB571A, filled histogram) or isotype control antibody (IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CX3CL1/Fractalkine in HEK293 cells transfected with mouse CX3CL1 and eGFP cells by Flow Cytometry.
HEK293 cells transfected with mouse CX3CL1 and eGFP was stained with eGFP and either (A) Rat Anti-Mouse CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # FAB571A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CX3CL1/Fractalkine APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line and HEK293 cells transfected with mouse CX3CL1 and eGFP
Sample: bEnd.3 mouse endothelioma cell line and HEK293 cells transfected with mouse CX3CL1 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CX3CL1/Fractalkine
CX3CL1, also known as Fractalkine, is a type I transmembrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. Although membrane-bound, it can also be released as a soluble molecule upon proteolysis at a membrane proximal site.
Alternate Names
FKN, Fractalkine, Neurotactin
Gene Symbol
CX3CL1
Additional CX3CL1/Fractalkine Products
Product Documents for Mouse CX3CL1/Fractalkine APC-conjugated Antibody
Product Specific Notices for Mouse CX3CL1/Fractalkine APC-conjugated Antibody
For research use only
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