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Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4531

R&D Systems, part of Bio-Techne
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MAB4531-100
MAB4531-500
MAB4531-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization, Western Blot

Cited:

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 124014

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1
Arg20-Lys96
Accession # P12850

Specificity

Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, rrCXCL5, or recombinant porcine CXCL8.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.

Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND50 is typically 1-5 µg/mL.

Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 aa 20-96 (Catalog # 453-KC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 aa 20-96.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

For research use only

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