Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4531
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 124014
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1
Arg20-Lys96
Accession # P12850
Arg20-Lys96
Accession # P12850
Specificity
Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha,
12/SDF-1 beta, 13, recombinant mouse CXCL2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinant
rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, rrCXCL5, or recombinant
porcine CXCL8.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody
Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.
Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND50 is typically 1-5 µg/mL.Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 aa 20-96 (Catalog # 453-KC)
under non-reducing conditions only
Sample: Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 aa 20-96 (Catalog # 453-KC)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha/KC/CINC-1 aa 20-96.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
UniProt
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody
Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody
For research use only
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