Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB452
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture), Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 40605
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL2/GRO beta/MIP-2/CINC-3
Ala28-Asn100
Accession # P10889
Ala28-Asn100
Accession # P10889
Specificity
Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta, rhCXCL3/GRO gamma, or recombinant mouse CXCL1/KC.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/MIP-2 Antibody.
Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.Applications for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 452-M2)
under non-reducing conditions only
Sample: Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 452-M2)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CXCL2/GRO beta/MIP-2/CINC-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3.
Mouse CXCL2/MIP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB452 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.
MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.
Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
UniProt
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Product Specific Notices for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
For research use only
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