Mouse CXCL7/Thymus Chemokine-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1091
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 159742
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1
Lys40-Tyr113
Accession # Q9EQI5
Lys40-Tyr113
Accession # Q9EQI5
Specificity
Detects mouse CXCL7/Thymus Chemokine‑1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, recombinant mouse (rm) CXCL1, 4, 6, 9, 10, 11, 12, 13, 15, 16, recombinant rat (rr) CXCL1, 2, 3, 5, recombinant porcine CXCL8, or recombinant cotton rat CXCL10 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL7/Thymus Chemokine-1 Antibody
Chemotaxis Induced by CXCL7/Thymus Chemokine-1 and Neutralization by Mouse CXCL7/Thymus Chemokine-1 Antibody.
Recombinant Mouse CXCL7/Thymus Chemokine-1 aa 48-109 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine-1 aa 48-109 (40 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL7/Thymus Chemokine-1 Monoclonal Antibody (Catalog # MAB1091). The ND50 is typically 2-8 µg/mL.Applications for Mouse CXCL7/Thymus Chemokine-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK) under non-reducing conditions only
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK) under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CXCL7/Thymus Chemokine-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine-1 aa 48-109.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/Thymus Chemokine-1
Alternate Names
Thymus Chemokine1
Gene Symbol
PPBP
UniProt
Additional CXCL7/Thymus Chemokine-1 Products
Product Documents for Mouse CXCL7/Thymus Chemokine-1 Antibody
Product Specific Notices for Mouse CXCL7/Thymus Chemokine-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...