Mouse CXCL7/Thymus Chemokine-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF793
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL7/Thymus Chemokine-1
Lys40-Tyr113
Accession # AAG36786
Lys40-Tyr113
Accession # AAG36786
Specificity
Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat CXCL7/Thymus Chemokine-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL7/Thymus Chemokine-1 Antibody
CXCL7/Thymus Chemokine-1 in Mouse Thymus.
CXCL7/Thymus Chemokine-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Chemotaxis Induced by CXCL7/Thymus Chemo-kine‑1 and Neutralization by Mouse CXCL7/Thymus Chemo-kine‑1 Antibody.
Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemo-kine-1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemo-kine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793). The ND50 is typically 0.8-4 µg/mL.Applications for Mouse CXCL7/Thymus Chemokine-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus
Sample: Perfusion fixed frozen sections of mouse thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK)
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK)
Neutralization
Measured by its ability to neutralize CXCL7/Thymus Chemokine-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine-1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/Thymus Chemokine-1
CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.
Alternate Names
Thymus Chemokine1
Gene Symbol
PPBP
UniProt
Additional CXCL7/Thymus Chemokine-1 Products
Product Documents for Mouse CXCL7/Thymus Chemokine-1 Antibody
Product Specific Notices for Mouse CXCL7/Thymus Chemokine-1 Antibody
For research use only
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