Mouse CXCL9/MIG Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # AF-492-NA
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
ELISA Development (Capture), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL9/MIG
Accession # P18340
Accession # P18340
Specificity
Detects mouse CXCL9/MIG in ELISAs. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL9/MIG Antibody
Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody.
Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.
Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence
mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CXCL9/MIG by Western Blot
mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CXCL9/MIG Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.
Mouse CXCL9/MIG Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL9/MIG
CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
Alternate Names
MIG
Gene Symbol
CXCL9
UniProt
Additional CXCL9/MIG Products
Product Documents for Mouse CXCL9/MIG Antibody
Product Specific Notices for Mouse CXCL9/MIG Antibody
For research use only
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