Mouse CXCL9/MIG Antibody

Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody.

Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND₅₀ is typically 6-20 µg/mL.

Detection of Mouse CXCL9/MIG by Immunocytochemistry/ Immunofluorescence

mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CXCL9/MIG by Western Blot

mTORC1 regulates Cxcl9 in osteoblasts.(a) Representative images of in situ hybridization of Cxcl9 mRNA in conjunction with immunostaining of Runx2 in femur sections of 12-week-old male mice bone. Boxed area is enlarged in the bottom right corner. Cxcl9+ osteoblasts out of total osteoblasts were also quantified. Scale bar, 50 μm. n=9 per group. (b) Representative photomicrographs of immunostaining of CXCR3 in CD31+ ECs in bone marrow and quantitative analysis of CXCR3+ ECs out of total ECs in 12-week-old male mice bone. Scale bar, 50 μm. n=9 per group. (c) Representative photomicrographs of immunostaining of CXCR3 in cultured HUVECs. Scale bar, 100 μm. (d) Cxcl9 concentrations assessed by ELISA in bone marrow (BM) and serum. n=5 per group. (e) Quantitative PCR analysis of Cxcl9 mRNA in primary osteoblasts. (f) Western blot of Cxcl9 in primary osteoblasts. (g) Concentrations of Cxcl9 in CM of primary osteoblasts assessed by ELISA. n=5 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms13885), licensed under a CC-BY license. Not internally tested by R&D Systems.