BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 Monoclonal Antibody (Catalog # MAB2145) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

Q9EQ16

Additional CXCR6 Products

Product Documents for Mouse CXCR6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CXCR6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CXCR6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2145

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CXCR6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CXCR6 Antibody

Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry.