Mouse Cystatin B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1409
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 227818
Product Specifications
Immunogen
E. coli-derived recombinant mouse Cystatin B
Met2-Phe98
Accession # Q62426
Met2-Phe98
Accession # Q62426
Specificity
Detects mouse Cystatin B in direct ELISAs and Western blots. In Western blots, 20‑25% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin D, S, SA, SN, recombinant mouse (rm) Cystatin A, E/M, rmFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Cystatin B Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin B (Catalog # 1409‑PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin B (Catalog # 1409‑PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Mouse Cystatin B (Catalog # 1409-PI)
Sample: Recombinant Mouse Cystatin B (Catalog # 1409-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin B
Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
- Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
- Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.
Alternate Names
CSTB, Stefin B
Gene Symbol
CSTB
UniProt
Additional Cystatin B Products
Product Documents for Mouse Cystatin B Antibody
Product Specific Notices for Mouse Cystatin B Antibody
For research use only
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