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Mouse Decorin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1060

R&D Systems, part of Bio-Techne
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AF1060
AF1060-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Simple Western, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654

Specificity

Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Decorin Antibody

Detection of Mouse Decorin antibody by Western Blot.

Detection of Mouse Decorin by Western Blot.

Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Decorin antibody by Simple WesternTM.

Detection of Mouse Decorin by Simple WesternTM.

Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Decorin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c., dorsal root ganglion)

Simple Western

10 µg/mL
Sample: Mouse adipose tissue and mouse colon tissue

Western Blot

0.5 µg/mL
Sample: Mouse adipose tissue and mouse colon tissue

Mouse Decorin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)
  • Standard: Recombinant Mouse Decorin Protein, CF (Catalog # 1060-DE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.8 using AF1060 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

References

  1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Gene Symbol

DCN

UniProt

Additional Decorin Products

Product Documents for Mouse Decorin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Decorin Antibody

For research use only

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