CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse DMBT1 Monoclonal Antibody (Catalog # MAB5915, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse DMBT1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMBT1

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted ~340 kDa, 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Long Name

Deleted in Malignant Brain Tumors 1

Alternate Names

CRP-ductin, Ebnerin, GP340, Hensin, Muclin, SAG, Vomeroglandin

Entrez Gene IDs

1755 (Human); 12945 (Mouse); 170568 (Rat)

Gene Symbol

DMBT1

UniProt

Q60997

Additional DMBT1 Products

Product Documents for Mouse DMBT1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse DMBT1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse DMBT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5915

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all DMBT1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse DMBT1 Antibody

Detection of DMBT1 in CMT‑93 Mouse Cell Line by Flow Cytometry.