Mouse DMBT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5915

R&D Systems, part of Bio-Techne
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AF5915
AF5915-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all DMBT1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse DMBT1
Trp215-Gly420
Accession # Q60997

Specificity

Detects mouse DMBT1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse DMBT1 Antibody

Detection of Mouse DMBT1 antibody by Western Blot.

Detection of Mouse DMBT1 by Western Blot.

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DMBT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5915) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DMBT1 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse DMBT1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMBT1

DMBT1 (Deleted in malignant brain tumor-1; also MUCLIN, Hensin, CRP-Ductin, and gp300/350) is a 230‑350 kDa member of the DMBT1 family of molecules. It is expressed by select cell types, including macrophages, intestinal epithelium, and pancreatic acinar cells. DMBT1 is both transmembrane (TM) and secreted, and possesses multiple activities that may be predicated on multimerization. It drives epithelial differentiation, binds Gm+ and Gm- bacteria, and associates with cytoskeletal actin. Mouse DMBT1 is a type I TM glycoprotein 2085 amino acids (aa) in length. Its extracellular region contains eight SRCR repeats (aa 31‑980, plus 1510‑1610), five CUB domains (aa 1023‑1490, plus 1633‑1742), and a ZP segment (aa 1751‑1999). Multiple splice variants exist. There are deletions of aa 584‑733, 397‑534, 593‑731 and, 2032‑2085 (within the TM segment), plus an insertion of 11 aa after Asp29. Over aa 215‑420, mouse DMBT1 shares 75% aa identity with human DMBT1.

Long Name

Deleted in Malignant Brain Tumors 1

Alternate Names

CRP-ductin, Ebnerin, GP340, Hensin, Muclin, SAG, Vomeroglandin

Entrez Gene IDs

1755 (Human); 12945 (Mouse); 170568 (Rat)

Gene Symbol

DMBT1

UniProt

Q60997

Additional DMBT1 Products

Product Documents for Mouse DMBT1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse DMBT1 Antibody

For research use only

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