Mouse DPPII/QPP/DPP7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3436
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPII/QPP/DPP7
Asp34-Arg506
Accession # NP_114031
Asp34-Arg506
Accession # NP_114031
Specificity
Detects mouse DPPII/QPP/DPP7 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human DPPII is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse DPPII/QPP/DPP7 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE)
Sample: Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE)
Neutralization
Measured by its ability to neutralize Recombinant Mouse DPPII/QPP/DPP7 (0.02 µg/mL, Catalog # 3436-SE) cleavage of the fluorogenic peptide substrate KP-Amc (10 µM). The Neutralization Dose (ND50) is typically 0.45 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPII/QPP/DPP7
Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a signal peptide (amino acids (aa) 1‑33) and a mature chain (aa 34‑506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.
References
- Araki, H. et al. (2001) J. Biochem. 129:279.
- Maes, M.-B. et al. (2005) Biochem. J. 386:315.
- Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
- Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Alternate Names
DPP7, QPP
Gene Symbol
DPP7
UniProt
Additional DPPII/QPP/DPP7 Products
Product Documents for Mouse DPPII/QPP/DPP7 Antibody
Product Specific Notices for Mouse DPPII/QPP/DPP7 Antibody
For research use only
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