Mouse EGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2028
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 262930
Product Specifications
Immunogen
E. coli-derived recombinant mouse EGF
Asn977-Arg1029
Accession # P01132
Asn977-Arg1029
Accession # P01132
Specificity
Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha, rhNRG1-beta 1, or rhTGF-alpha.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse EGF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse EGF (Catalog # 2028-EG)
Sample: Recombinant Mouse EGF (Catalog # 2028-EG)
Mouse EGF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
Epidermal Growth Factor (EGF) is the prototypic member of a family of growth factors that have EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
UniProt
Additional EGF Products
Product Documents for Mouse EGF Antibody
Product Specific Notices for Mouse EGF Antibody
For research use only
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