Mouse EMMPRIN/CD147 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF772
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652
Ala22-Arg209
Accession # NP_001070652
Specificity
Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse EMMPRIN/CD147 Antibody
Detection of Mouse EMMPRIN/CD147 by Western Blot.
Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse EMMPRIN/CD147 by Simple WesternTM.
Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse EMMPRIN/CD147 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse thymocytes
Sample: Mouse thymocytes
Simple Western
2 µg/mL
Sample: Mouse brain (cortex) tissue and mouse heart tissue
Sample: Mouse brain (cortex) tissue and mouse heart tissue
Western Blot
0.2 µg/mL
Sample: Mouse brain tissue, mouse heart tissue, and mouse small intestine tissue
Sample: Mouse brain tissue, mouse heart tissue, and mouse small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMMPRIN/CD147
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
UniProt
Additional EMMPRIN/CD147 Products
Product Documents for Mouse EMMPRIN/CD147 Antibody
Product Specific Notices for Mouse EMMPRIN/CD147 Antibody
For research use only
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