Mouse Endostatin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF570
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Porcine
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
ELISA Development, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Endostatin
His1105-Lys1288
Accession # AAA19787
His1105-Lys1288
Accession # AAA19787
Specificity
Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Endostatin Antibody
Endostatin in Mouse Intestine.
Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Endostatin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Endostatin
Sample: Recombinant Mouse Endostatin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.8 using AF570 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endostatin
Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.
Alternate Names
COL18A1
Gene Symbol
COL18A1
UniProt
Additional Endostatin Products
Product Documents for Mouse Endostatin Antibody
Product Specific Notices for Mouse Endostatin Antibody
For research use only
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