Mouse ENPP-7/Alk-SMase Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5160
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Ala22-Gln421
Accession # NP_001025462
Ala22-Gln421
Accession # NP_001025462
Specificity
Detects mouse ENPP‑7/Alk‑SMase in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse ENPP-7/Alk-SMase Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN)
Sample: Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ENPP-7/Alk-SMase
ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30 - 36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).
References
- Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
- Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
- Wu, J. et al. (2006) Biochem J. 394:299.
- Hertervig, E. et al. (1996) Cancer. 79:448.
- Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Alternate Names
Alk-SMase, ENPP7, NPP7
Gene Symbol
ENPP7
UniProt
Additional ENPP-7/Alk-SMase Products
Product Documents for Mouse ENPP-7/Alk-SMase Biotinylated Antibody
Product Specific Notices for Mouse ENPP-7/Alk-SMase Biotinylated Antibody
For research use only
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