Mouse ENPP-7/Alk-SMase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5160
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Ala22-Gln421
Accession # NP_001025462
Ala22-Gln421
Accession # NP_001025462
Specificity
Detects mouse ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse ENPP-7/Alk-SMase Antibody
ENPP‑7/Alk-SMase in intestine.
ENPP-7/Alk-SMase was detected in perfusion fixed frozen sections of mouse intestine using Mouse ENPP-7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse ENPP-7/Alk-SMase by Western Blot.
Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse ENPP-7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP-7/Alk-SMase at approximately 60kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse ENPP-7/Alk-SMase Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Mouse small intestine tissue and rat small intestine tissue
Sample: Mouse small intestine tissue and rat small intestine tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ENPP-7/Alk-SMase
ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).
References
- Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
- Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
- Wu, J. et al. (2006) Biochem J. 394:299.
- Hertervig, E. et al. (1996) Cancer. 79:448.
- Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Alternate Names
Alk-SMase, ENPP7, NPP7
Gene Symbol
ENPP7
UniProt
Additional ENPP-7/Alk-SMase Products
Product Documents for Mouse ENPP-7/Alk-SMase Antibody
Product Specific Notices for Mouse ENPP-7/Alk-SMase Antibody
For research use only
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