Mouse Ephrin-A4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB569

R&D Systems, part of Bio-Techne
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MAB569
MAB569-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 132416

View all Ephrin-A4 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Ephrin-A4
Leu26-Gly176
Accession # O08542

Specificity

Detects mouse Ephrin-A4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Ephrin-A4 is observed and no cross-reactivity with recombinant mouse (rm) Ephrin-A1, -A2, -B1, -B2, rhEphrin-A3, -A5, or -B3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Ephrin-A4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Ephrin-A4 Fc Chimera (Catalog # 569-A4)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-4, EFNA4, EphrinA4, LERK-4

Entrez Gene IDs

1945 (Human); 13639 (Mouse)

Gene Symbol

EFNA4

UniProt

O08542

Additional Ephrin-A4 Products

Product Documents for Mouse Ephrin-A4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Ephrin-A4 Antibody

For research use only

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