What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5580A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse F4/80 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: Raw264.7 Mouse Monocyte/Macrophage Cell Line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB5580A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse F4/80 APC-conjugated Antibody

For research use only

Citations
Reviews

Protocols
FAQs

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Flow Cytometry

Mouse F4/80 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5580A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all F4/80 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse F4/80 APC-conjugated Antibody

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.