Mouse F4/80 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5580P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 521204

View all F4/80 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Specificity

Detects mouse F4/80/EMR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse F4/80 PE-conjugated Antibody

Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse F4/80 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: F4/80

F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta+ proinflammatory macrophages.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse F4/80 PE-conjugated Antibody

For research use only

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