Mouse FGFR2 (IIIb) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7161

R&D Systems, part of Bio-Techne
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MAB7161
MAB7161-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 133730

View all FGFR2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse FGF R2 isoforms

Specificity

Detects IIIb isoforms of mouse FGF R2 in direct ELISAs and Western blots. This antibody shows approximately 75% cross-reactivity with recombinant human (rh) FGF R2 (IIIb) isoforms and no cross-reactivity with the IIIc isoform of mouse or human FGF R2 or any isoforms of rhFGF R1, rmFGF R3, or rhFGF R4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse FGFR2 (IIIb) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse FGF R2 alpha (IIIb) Fc Chimera (Catalog # 5496-MF)
Recombinant Mouse FGF R2 beta (IIIb) Fc Chimera (Catalog # 708-MF)
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Mouse FGFR2 (IIIb) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FGFR2 (IIIb) Antibody

For research use only

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