Mouse FOLR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6936
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse FOLR1
Arg23-Ser232
Accession # P35846
Arg23-Ser232
Accession # P35846
Specificity
Detects mouse FOLR1 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human FOLR1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse FOLR1 Antibody
FOLR1 in HeLa Human Cell Line.
FOLR1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FOLR1 in Mouse Kidney.
FOLR1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse FOLR1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
0.3-15 µg/mL
Sample:
Sample:
Perfusion fixed frozen sections of mouse kidney
Reviewed Applications
Read 1 review rated 5 using AF6936 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR1
Folate receptor 1 (FOLR1; also folate receptor alpha and folate binding protein 1/FOLBP1) is a 40-50 kDa member of the folate receptor family. FOLR1 is expressed in reticulocytes, retinal Muller cells and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The mouse FOLR1 preproprecursor is 255 amino acid (aa) in length. It contains a GPI-linked glycoprotein that is 208 aa in size (aa 25-232), and which possesses three potential N-linked glycosylation sites. There is one potential isoform variant that shows a 105 aa substitution for aa 110-255. Over aa 23-232, mouse FOLR1 shares 83% and 93% aa sequence identity with human and rat FOLR1, respectively.
Long Name
Folate Receptor 1
Alternate Names
FBP, Folbp1, FR-alpha, MOv18
Gene Symbol
FOLR1
UniProt
Additional FOLR1 Products
Product Documents for Mouse FOLR1 Antibody
Product Specific Notices for Mouse FOLR1 Antibody
For research use only
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