Mouse G-CSF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-414-NA
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse G-CSF (R&D Systems, Catalog # 414-CS)
Val31-Ala208
Accession # P09920
Val31-Ala208
Accession # P09920
Specificity
Detects mouse G-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human G‑CSF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse G-CSF Antibody
Cell Proliferation Induced by G-CSF and Neutralization by Mouse G-CSF Antibody.
Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA). The ND50 is typically 0.0025-0.0075 µg/mL.
Mouse G‑CSF ELISA Standard Curve.
Recombinant Mouse G-CSF protein was serially diluted 2-fold and captured by Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Mouse G-CSF Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse G-CSF DuoSet ELISA Kit (Catalog # DY414) for convenient development of a sandwich ELISA or the Mouse G-CSF Quantikine ELISA Kit (Catalog # MCS00) for a complete optimized ELISA.
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse G-CSF (Catalog # 414-CS)
Sample: Recombinant Mouse G-CSF (Catalog # 414-CS)
Neutralization
Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cell line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.0025-0.0075 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3 using AF-414-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for Mouse G-CSF Antibody
Product Specific Notices for Mouse G-CSF Antibody
For research use only
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