Mouse G-CSF DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY414
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
31.2-2000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse G-CSF DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse G-CSF DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse G-CSF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse G-CSF DuoSet ELISA
Mouse G-CSF ELISA Standard Curve
Kit Contents for Mouse G-CSF DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
Mouse G-CSF
receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs
to the hematopoietin receptor superfamily. The mature protein is 812 amino
acids in length and contains a 601 aa extracellular region, a 24 aa
transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular
region contains multiple modules, including an N-terminal Ig-like domain, a
cytokine receptor homology domain, and three fibronectin type III domains.
Based on crystallographic study, G-CSF receptor forms a complex with the ligand
in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity.
Cells known to express G-CSF R include monocytes and neutrophils,
megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic
progenitors, trophoblasts, endothelial cells and various tumor cell types.
G-CSF is an
important regulator for granulopoiesis in vivo. It has been demonstrated that
G-CSF can support the growth of multi-lineage hematopoietic progenitor cells
without influencing their commitment to the myeloid lineage and mobilize
hematopoietic progenitor cells from the bone marrow into the bloodstream. On
mature neutrophils, G-CSF may regulate neutrophil survival by controlling their
rate of apoptosis. G-CSF has also been shown to enhance the functional capacity
of mature neutrophils. As a consequence of its effects on hematopoietic
progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the
presence M-CSF. Within the peripheral blood stem cell population mobilized with
G-CSF, selective increases in the number of T helper 2-inducing dendritic cells
are found.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
Additional G-CSF Products
Product Documents for Mouse G-CSF DuoSet ELISA
Product Specific Notices for Mouse G-CSF DuoSet ELISA
For research use only
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