Mouse GDF-3 Propeptide Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3719

R&D Systems, part of Bio-Techne
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MAB3719
MAB3719-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 356613

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Product Summary for Mouse GDF-3 Propeptide Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GDF-3 Propeptide
Ser23-Lys250
Accession # Q3ZB19

Specificity

Detects mouse GDF-3 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) GDF-1, rmGDF-1pro, rmGDF-3, rmGDF-5, rmGDF-6, rmGDF-7, rmGDF-8, or rmGDF-9 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse GDF-3 Propeptide Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse GDF-3 (Catalog # 958-G3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-3

GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain (2‑4). In ES cells, it maintains pluripotency and influences early cell fate decisions (5, 6). For example, frog embryos injected with GDF-3 develop a secondary dorsal axis and deletion of mouse GDF-3 can produce defects in the anterior visceral endoderm of the pre‑gastrulation embryo (5, 6). In adipocytes, GDF-3 is induced by a high fat diet and promotes adipogenesis (3). GDF-3 has been reported to oppose BMP’s functions and to have a nodal-like activity in early development (1). The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. Most of GDF-3 is present as the prepro form, while the mature GDF-3 is presumably the secreted, active form (1). The mature protein contains the cysteine-knot structure that is conserved throughout family members. Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF-3 may exist as a non-covalent homodimer. Within the pro region, mouse and human GDF-3 share 65% aa sequence identity. Among family members, mature GDF-3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG‑1 (52% aa identity).

References

  1. Levine, A. J. and A. H. Brivanlou (2006) Cell Cycle 5:1069.
  2. McPherron, A. C. and S-J. Lee (1993) J. Biol. Chem. 268:3444.
  3. Wang, W. et al. (2004) Biochem. Biophys. Res. Comm. 321:1024.
  4. Hexige, S. et al. (2005) Neurosci. Lett. 389:83.
  5. Levine, A. J. and A. H. Brivanlou (2005) Development 133:209.
  6. Chen, C. et al. (2006) Development 133:319.

Long Name

Growth Differentiation Factor 3

Alternate Names

GDF3, Vgr-2

Entrez Gene IDs

9573 (Human); 14562 (Mouse)

Gene Symbol

GDF3

UniProt

Q3ZB19

Additional GDF-3 Products

Product Documents for Mouse GDF-3 Propeptide Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse GDF-3 Propeptide Antibody

For research use only

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