Mouse GFR alpha-like Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5728

R&D Systems, part of Bio-Techne
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MAB5728-100
MAB5728-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 1045708

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Product Summary for Mouse GFR alpha-like Antibody

Immunogen

Chinese Hamster Ovary cell line CHO-derived mouse GFR alpha‑like
Gln20-Gly349
Accession # Q6SJE0-1

Specificity

Detects mouse GFR alpha‑like in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse GFR alpha-like Antibody

GFR alpha-like in HEK293 Human Cell Line Transfected.

GFR alpha-like was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rat Anti-Mouse GFR alpha-like Monoclonal Antibody (Catalog # MAB5728) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse GFR alpha-like Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References


  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Yang, L. et al. (2017) Nature Medicine 23:1158.
  3. Mullican, S. et al. (2017) Nature medicine 23:1150.

  4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha-like

Alternate Names

GFR alpha like, GFRAL, GRAL

Entrez Gene IDs

389400 (Human); 404194 (Mouse); 501023 (Rat)

Gene Symbol

GFRAL

UniProt

Q6SJE0-1

Additional GFR alpha-like Products

Product Documents for Mouse GFR alpha-like Antibody

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Certificate of Analysis

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Product Specific Notices for Mouse GFR alpha-like Antibody

For research use only

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