Mouse Glycoprotein V/CD42d Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6990

R&D Systems, part of Bio-Techne
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AF6990
AF6990-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Glycoprotein V/CD42d Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Glycoprotein V/CD42d
Gln17-Gly522
Accession # NP_032174

Specificity

Detects mouse Glycoprotein V/CD42d in direct ELISAs and Western blots.  

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Glycoprotein V/CD42d Antibody

Detection of Mouse Glycoprotein V/CD42d antibody by Western Blot.

Detection of Mouse Glycoprotein V/CD42d by Western Blot.

Western blot shows lysates of mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Glycoprotein V/ CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Glycoprotein V/CD42d at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glycoprotein V/CD42d antibody in Mouse Platelets by Immunocytochemistry (ICC).

Glycoprotein V/CD42d in Mouse Platelets.

Glycoprotein V/CD42d was detected in immersion fixed mouse platelets using Sheep Anti-Mouse Glycoprotein V/CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Glycoprotein V/CD42d Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse platelets

Western Blot

1 µg/mL
Sample: Mouse platelets

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycoprotein V/CD42d

GP-V (platelet glycoprotein V; also CD42d) is a 78-88 kDa member of the leucine-rich (LR) repeat family of proteins. It is expressed on the surface of platelets where it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for both thrombin and von Willebrand factor. In addition, GP-V is normally a receptor for collagen. Following receptor complex activation due to thrombin cleavage of GP-V, the collagen-binding activity of GP-V is lost, limiting thrombus growth at sites of clot formation. Mature mouse GP-V is a 551 amino acid (aa) type I transmembrane glycoprotein. In its 506 aa extracellular domain (aa 17-522), GP-V contains 16 LR repeats (aa 17-474), and terminates with a short 24 aa cytoplasmic tail. Based on human, mouse GP-V might be naturally cleaved by thrombin and ADAM17, generating soluble fragments that range from 70-82 kDa in size. Over aa 17-522, mouse GP-V shares 83% and 70% aa sequence identity with rat and human GP-V, respectively.

Alternate Names

CD42d, GP5, GPV

Entrez Gene IDs

2814 (Human); 14729 (Mouse); 25259 (Rat)

Gene Symbol

GP5

UniProt

NP_032174

Additional Glycoprotein V/CD42d Products

Product Documents for Mouse Glycoprotein V/CD42d Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Glycoprotein V/CD42d Antibody

For research use only

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