Mouse HAI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1141
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Canine
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Specificity
Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse HAI-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse HAI-1 (Catalog # 1141-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse HAI-1 (Catalog # 1141-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse HAI-1 (Catalog # 1141-PI)
Sample: Recombinant Mouse HAI-1 (Catalog # 1141-PI)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-1
Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
References
- Denda, et al. (2002) J. Biol. Chem. 277:14053.
- Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
- Kataoka, et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Alternate Names
HAI1, SPINT1
Gene Symbol
SPINT1
UniProt
Additional HAI-1 Products
Product Documents for Mouse HAI-1 Antibody
Product Specific Notices for Mouse HAI-1 Antibody
For research use only
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