Mouse HAI-2 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1107
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-2B
Arg30-Lys140
Accession # NP_001076017
Arg30-Lys140
Accession # NP_001076017
Specificity
Detects mouse HAI-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human HAI-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse HAI-2 Ectodomain Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver
Sample: Perfusion fixed frozen sections of mouse liver
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse HAI-2 Ectodomain
Sample: Recombinant Mouse HAI-2 Ectodomain
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-2
Two alternatively spliced forms of HAI-2 are found in human tissues. HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain. Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trypsin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa.
Three alternatively spliced forms of HAI-2 have been found in mouse tissues. HAI-2A is a minor form expressed in mouse tissues. HAI-2B, with residues 37 through 93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin.
Long Name
HGF Activator Inhibitor Type 2
Alternate Names
HAI2
Gene Symbol
SPINT2
UniProt
Additional HAI-2 Products
Product Documents for Mouse HAI-2 Ectodomain Antibody
Product Specific Notices for Mouse HAI-2 Ectodomain Antibody
For research use only
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