Mouse HSP10/EPF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2584
Key Product Details
Species Reactivity
Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse HSP10/EPF
Ala2-Asp102
Accession # Q64433
Ala2-Asp102
Accession # Q64433
Specificity
Detects mouse HSP10/EPF in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse HSP10/EPF Antibody
Detection of Mouse HSP10/EPF by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Mouse HSP10/EPF by Simple WesternTM.
Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse HSP10/EPF Antibody
Application
Recommended Usage
Simple Western
25 µg/mL
Sample: DA3 mouse myeloma cell line
Sample: DA3 mouse myeloma cell line
Western Blot
0.5 µg/mL
Sample: DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line
Sample: DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP10/EPF
The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10.
Long Name
Heat Shock 10 kDa Protein 1
Alternate Names
Chaperonin 10 Homolog, EPF, GroES, HSPE1
Gene Symbol
HSPE1
UniProt
Additional HSP10/EPF Products
Product Documents for Mouse HSP10/EPF Antibody
Product Specific Notices for Mouse HSP10/EPF Antibody
For research use only
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