Mouse ICAM-1/CD54 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB796P

R&D Systems, part of Bio-Techne
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FAB796P
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MAB796
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 166623

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Gln28-Asn485
Accession # P13597

Specificity

Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse ICAM-1/CD54 PE-conjugated Antibody

Detection of ICAM-1/CD54 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse ICAM-1/CD54 PE-conjugated Monoclonal Antibody (Catalog # FAB796P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ICAM-1/CD54 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ICAM-1/CD54

ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an 85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458 aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety of cells, including neurons, dendritic cells, fibroblasts, skeletal muscle myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and vascular endothelial cells. On the cell surface, it exists primarily as an "intermediate" between a homodimeric and monomeric state. The homodimeric pairing does not seem to offer a distinct advantage with respect to ligand binding. On the other hand, the degree of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation associated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alphaL beta2), Mac-1 ( alphaM beta2), CD11c/CD18, fibrinogen, hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF, acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white cell trafficking (leukocyte binding to endothelium), and is also reported to play a key role in the establishment of CD8+ T cell memory, serving as a link between mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54% aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1 listed in SwissProt that shows a 28 aa substitution for aa 1-34.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

UniProt

P13597

Additional ICAM-1/CD54 Products

Product Documents for Mouse ICAM-1/CD54 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ICAM-1/CD54 PE-conjugated Antibody

For research use only

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