Mouse IFN-alpha Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # 32100-1
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Product Specifications
Immunogen
E. coli-derived recombinant mouse IFN-alpha A
Specificity
Mouse IFN-alpha
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Applications for Mouse IFN-alpha Antibody
Application
Recommended Usage
Neutralization
One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
N/A
Formulation
Neat serum diluted in phosphate buffered saline (PBS)
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN-alpha Antibody
Product Specific Notices for Mouse IFN-alpha Antibody
For research use only
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