Mouse IFN-alpha / beta R2 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1083F

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

View all IFN-alpha/beta R2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7

Specificity

Detects mouse IFN‑ alpha/ beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IFN-alpha / beta R2 Fluorescein-conjugated Antibody

Detection of IFN-a/ beta R2 antibody in A20 Mouse Cell Line antibody by Flow Cytometry.

Detection of IFN‑ alpha/ beta R2 in A20 Mouse Cell Line by Flow Cytometry.

A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IFN-a/ beta R2 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1083F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IFN-alpha / beta R2 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A20 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name

Interferon alpha/beta Receptor 2

Alternate Names

IFN-aR2, IFN-bR2, IFNAR2, IFNbR2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Gene Symbol

IFNAR2

UniProt

Q9D1R7

Additional IFN-alpha/beta R2 Products

Product Documents for Mouse IFN-alpha / beta R2 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN-alpha / beta R2 Fluorescein-conjugated Antibody

For research use only

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