Mouse IGSF8/CD316 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3117
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316
Ala25-Thr577
Accession # NP_536344
Ala25-Thr577
Accession # NP_536344
Specificity
Detects mouse IGSF8/CD316 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IGSF8/CD316 Biotinylated Antibody
IGSF8/CD316 in Neuro‑2A Mouse Cell Line.
IGSF8/CD316 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse IGSF8/CD316 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Neuro-2A mouse neuroblastoma cell line
Sample: Neuro-2A mouse neuroblastoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Neuro2A mouse neuroblastoma cell line
Sample: Immersion fixed Neuro2A mouse neuroblastoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IGSF8
Sample: Recombinant Mouse IGSF8
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGSF8/CD316
Long Name
Immunoglobulin Superfamily, Member 8
Alternate Names
CD316, CD81P3, EWI2, KCT4, PGRL
Gene Symbol
IGSF8
UniProt
Additional IGSF8/CD316 Products
Product Documents for Mouse IGSF8/CD316 Biotinylated Antibody
Product Specific Notices for Mouse IGSF8/CD316 Biotinylated Antibody
For research use only
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