Mouse IKK beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7155

R&D Systems, part of Bio-Techne
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MAB7155
MAB7155-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 725818

View all IKK beta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IKK beta
Val530-Asp757
Accession # O88351

Specificity

Detects mouse IKK beta in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IKK beta Antibody

Detection of Mouse IKK beta antibody by Western Blot.

Detection of Mouse IKK beta by Western Blot.

Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IKK beta Monoclonal Antibody (Catalog # MAB7155) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IKK beta Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: BaF3 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappaB kinase beta (IKK beta) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF kappaB. Like IKK alpha, IKK beta contains kinase (aa 15-300), leucine zipper (aa 458‑479), and helix-loop-helix (aa 605-644) domains. NF kappaB-inducing kinase (NIK) phosphorylates and activates IKK alpha/IKK beta heterodimers. Within amino acids 530‑757, mouse IKKb shares 89% and 96% aa sequence identity with human and rat IKKb, respectively.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse); 84351 (Rat)

Gene Symbol

IKBKB

UniProt

O88351

Additional IKK beta Products

Product Documents for Mouse IKK beta Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IKK beta Antibody

For research use only

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