Mouse IL-10 R beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5368
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta
Ile21-Ser220
Accession # Q61190
Ile21-Ser220
Accession # Q61190
Specificity
Detects mouse IL-10 R beta in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IL‑10 R beta is observed and less than 1% cross-reactivity with rhIL-10 R and recombinant mouse IL-10 R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IL-10 R beta Antibody
Detection of IL‑10 R beta in A20 Mouse Cell Line by Flow Cytometry.
A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).Applications for Mouse IL-10 R beta Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10 R beta
IL-10 R beta (Interleukin 10 receptor beta; also IL‑10 R2, CD210b and CRF2-4) is a 60‑65 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20‑220) that possesses two fibronectin type III domains (aa 22‑107 and 111‑208). There is one alternate start site at Met20. Over aa 21‑220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.
Long Name
Interleukin 10 Receptor beta
Alternate Names
CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB
Gene Symbol
IL10RB
UniProt
Additional IL-10 R beta Products
Product Documents for Mouse IL-10 R beta Antibody
Product Specific Notices for Mouse IL-10 R beta Antibody
For research use only
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