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Mouse IL-12 R beta1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1998P

R&D Systems, part of Bio-Techne
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FAB1998P-100
FAB1998P-025

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta1
Val32-Glu561
Accession # Q60837

Specificity

Detects mouse IL-12 R beta1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant human IL-12 R beta1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IL-12 R beta1 PE-conjugated Antibody

Detection of IL-12 R beta1 antibody in D10.G4.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of IL‑12 R beta1 in D10.G4.1 Mouse Cell Line by Flow Cytometry.

D10.G4.1 mouse helper T cell line was stained with Goat Anti-Mouse IL-12 R beta1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1998P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL-12 R beta1 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: D10.G4.1 mouse helper T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12 R beta 1

IL-12 R beta1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta1, respectively. It shares 16%-21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta1 with either IL-12 R beta2 or IL-23 R (4-6). IL-12 R beta2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8-10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta2 (13, 14).

References

  1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
  2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
  4. Parham, C. et al. (2002) J. Immunol. 168:5699.
  5. Wu, C. et al. (1997) J. Immunol. 159:1658.
  6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
  8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
  9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
  10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
  11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
  12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
  13. Shimozato, O. et al. (2006) Immunology 117:22.
  14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

Additional IL-12 R beta 1 Products

Product Documents for Mouse IL-12 R beta1 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-12 R beta1 PE-conjugated Antibody

For research use only

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