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Mouse IL-15R alpha Alexa Fluor® 750-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5511S

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 888220

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
Gly33-Lys205
Accession # Q60819

Specificity

Detects mouse IL-15 R alpha in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse IL-15R alpha Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: C57BL/6 mouse bone marrow cells differentiated into dendritic cells by treatment with 20 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) and LPS
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL-15 R alpha) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non‑lymphoid cells. IL-15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gammac, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

Additional IL-15R alpha Products

Product Documents for Mouse IL-15R alpha Alexa Fluor® 750-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-15R alpha Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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