C57BL/6 mouse bone marrow cells treated with 20 ng/mL Recombinant Mouse GM-CSF (Catalog # 415-ML) for 5 days, 1 µg/mL LPS on day 6, and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB5511A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL-15R alpha APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: C57BL/6 mouse bone marrow cells treated with 20 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) for 5 days, 1 μg/mL LPS on day 6, and harvested on day 7

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL-15 R alpha) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gammac, through the heterodimeric complex of IL-2 receptors beta and gamma common, and through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

Q60819

Additional IL-15R alpha Products

Product Documents for Mouse IL-15R alpha APC-conjugated Antibody

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COA

SDS

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Product Specific Notices for Mouse IL-15R alpha APC-conjugated Antibody

For research use only

Citations
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Flow Cytometry

Mouse IL-15R alpha APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5511A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IL-15R alpha APC-conjugated Antibody

Detection of IL‑15 R alpha in C57BL/6 Mouse Bone Marrow Cells by Flow Cytometry.