Mouse IL-18 R alpha/IL-1 R5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB12161
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunoprecipitation, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 112624
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-18 R alpha
Ser19-Gly326
Accession # Q61098
Ser19-Gly326
Accession # Q61098
Specificity
Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-18 R alpha/IL-1 R5 Antibody
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha/IL‑1 R5 Antibody.
In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.Applications for Mouse IL-18 R alpha/IL-1 R5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse IL-18 R alpha/IL-1 R5
Sample: Recombinant Mouse IL-18 R alpha/IL-1 R5
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
Long Name
Interleukin 18 Receptor alpha
Alternate Names
CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1
Gene Symbol
IL18R1
UniProt
Additional IL-18 R alpha/IL-1 R5 Products
Product Documents for Mouse IL-18 R alpha/IL-1 R5 Antibody
Product Specific Notices for Mouse IL-18 R alpha/IL-1 R5 Antibody
For research use only
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