Mouse IL-18 R beta/IL-1 R7 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1520

R&D Systems, part of Bio-Techne
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BAF1520
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AF199
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-18 R beta/IL-1 R7 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R beta/IL‑1 R7
Phe20-Glu356
Accession # Q9Z2B1

Specificity

Detects mouse IL-18 R beta in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-18 R beta is observed, 10% cross-reactivity with rhIL-1 R9 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) IL-1 R8, recombinant rat IL-1 R6, rhIL-1 R3, rmIL-1 R1, rmIL-1 RII, and rmIL-18 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IL-18 R beta/IL-1 R7 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL-18 R beta/IL-1 R7
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18R alpha (IL-1 R5) and IL-18R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18R alpha by itself binds IL-18 with low-affinity and IL-18R beta does not bind IL-18 in vitro, co-expression of IL-18R alpha and IL-18R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a aa signal peptide, a aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18R beta parallels that of IL-18R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Alternate Names

AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Gene Symbol

IL18RAP

UniProt

Q9Z2B1

Additional IL-18 R beta/IL-1 R7 Products

Product Documents for Mouse IL-18 R beta/IL-1 R7 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse IL-18 R beta/IL-1 R7 Biotinylated Antibody

For research use only

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