Mouse IL-21 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF594
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Bovine
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Neutralization, Western Blot
Cited:
ELISA Capture, ELISA Development, ELISpot Development, Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-21 Antibody
IL-21 in Mouse Splenocytes.
IL-21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-21 and Neutralization by Mouse IL-21 Antibody.
Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 0.2-2 µg/mL.Applications for Mouse IL-21 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed concanavalin A-stimulated mouse splenocytes
Sample: Immersion fixed concanavalin A-stimulated mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Neutralization
Measured by its ability to neutralize IL‑21-induced proliferation in the NK-92 human natural killer lymphoma cell line. The Neutralization Dose (ND50) is typically 0.2-2 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑21.
Mouse IL-21 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF594 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gammac) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) PNAS 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Alternate Names
CVID11, IL21, Za11
Gene Symbol
IL21
UniProt
Additional IL-21 Products
Product Documents for Mouse IL-21 Antibody
Product Specific Notices for Mouse IL-21 Antibody
For research use only
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