Mouse IL-21R Antibody
R&D Systems, part of Bio-Techne | Catalog # AF596
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human IL-21 R is observed, and less than 1% cross-reactivity will recombinant mouse IL-2 R beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IL-21R Antibody
Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry.
A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-21 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF596, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
IL-10 in Mouse Splenocytes.
IL-10 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells<.>Applications for Mouse IL-21R Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-21 R Subunit Fc Chimera (Catalog # 596-MR)
Sample: Recombinant Mouse IL-21 R Subunit Fc Chimera (Catalog # 596-MR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: IL-21R
The interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor) is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 R is a 529 amino acid (aa) residue protein with a 19 aa signal peptide, a 217 aa extracellular domain, an 18 aa transmembrane domain, and a 275 aa cytoplasmic domain. Mouse and human IL-21 R share 62% aa identity. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B and natural killer lineage. Although not fully elucidated, the IL-2 R gamma ( gammac) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and natural killer cell maturation.
References
- Parrish-Novak, J. et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21 Receptor
Alternate Names
CD360, IL-21 R, IL21R, NILR
Entrez Gene IDs
Gene Symbol
IL21R
UniProt
Additional IL-21R Products
Product Documents for Mouse IL-21R Antibody
Product Specific Notices for Mouse IL-21R Antibody
For research use only
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