Mouse IL-23R APC-conjugated Antibody

Name: Mouse IL-23R APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB16861A
Rating: 1 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # FAB16861A

(1)

Citations (2)

Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R Gly24-Asp372 Accession # Q8K4B4
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Rat IgG₁ Clone # 753317
Specificity:	Detects endogenous mouse IL-23 R by flow cytometry.

View Full Technical Details for Mouse IL-23R APC-conjugated Antibody

Technical Data
Citations / Reviews (1)

Applications for Mouse IL-23R APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-23R APC-conjugated Antibody

	Flow Cytometry Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 10 µg/mL Anti-CD3 (Catalog # MAB484), 5 µg/mL Anti-CD28 (Catalog # AF483), 10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B), 20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML), 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML) for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB16861A) or (B) Rat IgG₁Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
	Flow Cytometry Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB16861A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺ CD45RB^low memory T cells but at much lower levels by mouse CD4⁺ CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

Item	Value
References	Oppmann, B. et al. (2000) Immunity 13:715. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49. Parham, C. et al. (2002) J. Immunol. 168:5448. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name	Interleukin 23 Receptor
Entrez Gene IDs	149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)
Alternate Names	IBD17, IL-23 R, IL-23 receptor, IL-23R, IL23R, interleukin 23 receptor, interleukin-23 receptor

Mouse IL-23R APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB16861A

Key Product Details

Applications for Mouse IL-23R APC-conjugated Antibody

Scientific Data Examples for Mouse IL-23R APC-conjugated Antibody

Flow Cytometry

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: IL-23R

Product Documents for Mouse IL-23R APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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