Mouse IL-23R PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB16861P

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 753317

View all IL-23R Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
Gly24-Asp372
Accession # Q8K4B4

Specificity

Detects endogenous mouse IL-23 R by flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IL-23R PE-conjugated Antibody

Detection of IL-23 R antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with 10 µg/mL Anti-CD3 (Catalog # MAB484), 5 µg/mL Anti-CD28 (Catalog # AF483), 10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B), 20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML), 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML) for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB16861P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-23 R antibody in HEK293 Human Cell Line Transfected with Mouse IL-23 R antibody by Flow Cytometry.

Detection of IL-23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB16861P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL-23R PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with Anti-CD3 (Catalog # MAB484), Anti-CD28 (Catalog # AF483), Recombinant Mouse TGF-beta 1 (Catalog # 100-B), Recombinant Mouse IL-23 (Catalog # 1887-ML), Recombinant Mouse IL-6 (Catalog # 406-ML), and Recombinant Mouse IL-1 beta (Catalog # 401-ML) to induce T17 activation or HEK293 human embryonic kidney cell line transfected with mouse IL-23 R
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5448.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23 Receptor

Alternate Names

IL-23 R, IL23R

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Gene Symbol

IL23R

UniProt

Q8K4B4

Additional IL-23R Products

Product Documents for Mouse IL-23R PE-conjugated Antibody

Product Datasheets

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Product Specific Notices for Mouse IL-23R PE-conjugated Antibody

For research use only

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