Mouse IL-36 alpha/IL-1F6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB22972

R&D Systems, part of Bio-Techne
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MAB22972-100
MAB22972-SP
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 275302

View all IL-36 alpha/IL-1F6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑36 alpha/IL‑1F6
Met1-His160
Accession # Q9JLA2

Specificity

Detects mouse IL‑36 alpha/IL‑1F6 in direct ELISAs

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse IL-36 alpha/IL-1F6 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse IL‑36 alpha/IL‑1F6 Antibody (Catalog # AF2297). This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Mouse interleukin 1 family member #6 [IL-1F6; also named FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL‑1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta-strand, beta-trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 160 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5). It appears to be actively secreted (1). When found in cell lysates, it presents as an 18 kDa monomer (2). Mouse to human, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 29% aa identical to IL-1ra, and 32%, 32%, 34%, 41%, and 28% aa identical to IL-1 beta, IL-1F5, F8, F9, and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL‑1F6 may signal in similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Gene Symbol

IL36A

UniProt

Q9JLA2

Additional IL-36 alpha/IL-1F6 Products

Product Documents for Mouse IL-36 alpha/IL-1F6 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-36 alpha/IL-1F6 Antibody

For research use only

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