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Mouse IL-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF-405-NA

R&D Systems, part of Bio-Techne
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AF-405-NA
AF-405-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-5

Specificity

Detects mouse IL‑5 in direct ELISAs and Western blots. Neutralizes the biological activity of recombinant mouse IL-5, but will not neutralize the biological activity of recombinant human IL-5. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-5 Antibody

Cell Proliferation Induced by IL-5 and Neutralization by Mouse IL-5 Antibody.

Cell Proliferation Induced by IL-5 and Neutralization by Mouse IL-5 Antibody.

Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-405-NA). The ND50 is typically 0.4-0.9 µg/mL.

Applications for Mouse IL-5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL-5 (Catalog # 405-ML)

Neutralization

Measured by its ability to neutralize IL-5-induced proliferation in the TF-1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.4-0.9 µg/mL in the presence of 1.25 ng/mL Recombinant Mouse IL-5.

Reviewed Applications

Read 1 review rated 4 using AF-405-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross-reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha-subunit and a non-binding common beta-subunit that is shared with the high-affinity receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Mouse IL-5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-5 Antibody

For research use only

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